Great Western Blotting Blocking Buffer from LI-COR

Johns Hopkins University School of Medicine
Department of Cell Biology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

LI-COR

Product Name:

Odyssey Blocking Buffer

Catalog Number:

927-50000

Our lab uses LI-COR imaging system for western blotting. We used Odyssey Blocking Buffer (TBS), which is a ready-to-use formulation in Tris-buffered saline that provides optimal blocking conditions for antibodies requiring TBS-based buffer systems

Experimental Design and Results Summary

Application

Western blotting

Starting Material

NC membrane or PVDF membrane

Tips

I always dilute the buffer with TBST at the ratio of 1:1.

Results Summary

Blocking works very well with this buffer and only minimal background could be detected.

DOI or PMID #

30194235

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Summary

The Good

Reliable, high quality, user friendly

The Bad

The price is high

The Bottom Line

I highly recommend this blocking buffer for Immunoblotting. It works very well.

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