OK Anti-human CD4 Antibody

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

V450 Mouse Anti-Human CD4

Catalog Number:

560345

Clone Number:

RPA-T4

Info: Supplier Page

Browse Similar Products: CD4 Flow Cytometry Antibodies

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. Here, we are just titrating a new anti-CD4 in human PBMCs.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs depleted of CD8 T cells

Primary Incubation

Anti-CD4 V450 1/50 (best dilution), 30', 4C

Blocking Agent

Fc block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometer

Results Summary

We could stain CD4 T cells best using this antibody at 1/50.

Image Gallery

Summary

The Good

It works

The Bad

Not the brightest option

The Bottom Line

It can be used

Share your experience with other scientists. Write a Review! »

Join the discussion