Good Option for Human CD38 Antibody

July 07, 2020

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Anti-human CD38 Antibody PE-Vio770

Catalog Number:

130-113-990

Clone Number:

IB6

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining plasmablasts (CD27+CD38+) in whole blood of a healthy donor (HD).

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Healthy donor human whole blood RBC lysed

Primary Incubation

anti CD38 PE-Vio770 (1/200), 30' 4C

Blocking Agent

Fc block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometer

Results Summary

We could stain PB (CD27+CD38+) in whole blood.

Image Gallery

Summary

The Good

It works

The Bad

Could be brighter

The Bottom Line

Good option to stain hCD38

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