Miltenyi Biotec
CD20 Antibody, anti-human, VioGreen
130-113-379
LT20
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining B cells (CD19+CD20+) in whole blood of a healthy donor (HD).
Flow Cytometry
Human whole blood (Healthy Donor)
Anti-human CD20 VioGreen (1/200), 30', 4C
10µL Fc Blocking Reagent, 10min 4C
N/A
Flow cytometer. VioGreen can be read in the BV510 channel.
As it is possible to observe in the figure, we could stain CD20+CD19+ B cells.
It works
It is not very bright
It works!