Usable Anti-Mouse IgM

June 18, 2020

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

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Company:

BioLegend

Product Name:

PE/Cyanine7 anti-mouse IgM Antibody

Catalog Number:

406513

Clone Number:

RMM-1

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining IgM expressing B cells (CD19+) in mouse spleen.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

Surface staining with anti-IgMPE-Cy7 (1/100), 20 min at 4C

Blocking Agent

Fc block (2.4G2 clone)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

We could stain IgM+ B cells that are almost all the B cells in a naive spleen. Also, some plasma cells are IgM+, while there are also switched plasma cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

It works

The Bad

It is not the brightest.

The Bottom Line

It is usable.

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