BioLegend
Brilliant Violet 510 anti-mouse/human CD45R/B220 Antibody
103248
RA3-6B2
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining B cells (CD19+B220+) and plasma cells (B220lowCD138hi).
Flow Cytometry
C57BL/6 spleen
surface staining with B220 BV510 (1/200) , 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometry
We could stain B cells (CD19+B220+) and plasma cells (B220loCD138hi).
It works well
Very good option to stain mouse B220.