BD Biosciences
PE Mouse Anti-Zap70 (Y319)/Syk (Y352)
557881
17A/P-ZAP70
Browse Similar Products: Syk Flow Cytometry Antibodies
Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of B cells and antibody secreting cells (ASC) in health and disease. In this experiment, we stained phopho-Syk before and after BCR stimulation.
Flow Cytometry
Isolated PBMCs from HD peripheral blood
Lyse/Fix buffer (BD Biosciences) 10' 37◦C
Fc Block
Anti-pSyk PE 1/10
N/A
Flow cytometer
B cells upregulate p-Syk upon BCR stimulation.
31616406
It works.
Could be cheaper.
Good option to stain p-Syk