Worked, but Not the Best Stain for Pax-6

Genetics
Emory University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend (Covance)

Product Name:

Pax6

Catalog Number:

PRB-278P

Clone Number:

Poly19013

Pax6 antibody does work but takes time to optimize

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Organoid Samples

Primary Incubation

1:500 overnight at 4C

Blocking Agent

5% donkey serum + 0.1% Triton X-100 in PBS

Secondary Incubation

1:1000 for 2 hours at room temperature

Tertiary Incubation

None

Detection

Fluorescent Microscope

Results Summary

Does work but stain was not as strong as we would have liked.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works fine

The Bad

Could stain better

The Bottom Line

I would recommend but it might take some optimization to get the best results

Share your experience with other scientists. Write a Review! »

Comments