TBR1 Antibody Worked Very Poorly

May 19, 2020

Genetics
Emory University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

TBR1

Catalog Number:

ab31940

Clone Number:

Polyclonal

TBR1 did not stain my sample, whereas other neuronal markers did stain the sample tissue

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Cortical Organoids

Primary Incubation

1:1500 overnight at 4C

Blocking Agent

5% Donkey serum + 0.1% Triton X-100 in PBS

Secondary Incubation

1:1000 for 2 hours at room temp

Tertiary Incubation

None

Detection

Fluorescent Microscope

Results Summary

Used a strong secondary that worked before, so that was not an issue, but the Tbr1 did not stain well at all

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Did get some signal

The Bad

Did not stain cell types

The Bottom Line

Worked very poorly for our human cortical organoid samples

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