This Anti-Rabbit Secondary Antibody Works Extremely Well

June 23, 2020

Genetics
Emory University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Goat anti-Rabbit Alexa Fluor 488

Catalog Number:

ab150081

Clone Number:

Polyclonal

Great secondary antibody!

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Cell Culture

Primary Incubation

1:1000 overnight at 4C

Blocking Agent

4% Donkey Serum + 0.1% Triton X-100 in PBS

Secondary Incubation

1:1000 for 2 hours at room temp

Tertiary Incubation

N/A

Detection

Fluorescent Microscope

Results Summary

The secondary antibody binds very strongly to the primary antibody with limited background.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Strong signal with limited background noise

The Bad

None

The Bottom Line

Great secondary antibody to use!

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