Good Anti-Human CD8 Antibody

June 16, 2020

BlueskyReddit
B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC/Fire™ 750 anti-human CD8 Antibody

Catalog Number:

344746

Clone Number:

SK1

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) in health and disease, although in this particular experiment, we are looking for CD3+CD8+ T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

Anti-CD8 APC-Fire750 1/50 15min 4C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

We could stain CD3+CD8+ T lymphocytes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

It works well

The Bad

N/A

The Bottom Line

It is a good option to stain human CD8

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