Deoxyribonuclease I Is Ideal for Digesting DNA to Remove Interference

June 03, 2020

BlueskyReddit
UAB
Radiation Oncology
Research Scientist

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Company:

Sigma-Aldrich

Product Name:

Deoxyribonuclease I from bovine pancreas

Catalog Number:

D5025

Human tumor specimens were dissociated with the Miltenyi Tumor Dissociation it. As there were concerns about sticky DNA adhering to viable cells and clogging the strainer, DNase I was employed to remove it.

Experimental Design and Results Summary

Application

Tumor Dissociation

Starting Material

Human cervical tumor

Tips

Make sure to carefully calculate the amount needed as the quantification is measured in units (or Kunitz units). PBS for dissolving DNase I works fine at 5 mg/mL.

Results Summary

No viscosity was apparent and the cell strainer did not get clogged when run the dissociated tumor cells through.

DOI or PMID #

N/A

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Summary

The Good

Can be dissolved in PBS

The Bad

Need to convert Kunitz (which is outdated now for enzyme activity)

The Bottom Line

DNase I from bovine pancreas is an effective enzyme for digesting DNA to help clear it and prevent obstruction of downstream analyses. It can be easily dissolved in PBS (however, this may not be stored for a very long time as enzyme activity may decrease dramatically after a few months in -20 degrees).

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