Average CX3CR1 Antibody for IF

June 18, 2020

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Anti-CX3CR1-PE, human

Catalog Number:

130-096-432

Clone Number:

2A9-1

The focus of this project was to investigate the pathogenesis of the Alzheimer's disease. This antibody was used to determine the CX3CR1 expression in astrocytes.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Brain

Primary Incubation

1:20; RT; 2 h

Blocking Agent

5 % FBS; RT, 30 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence signals (PE) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives specific signals on frozen-tissue sections with some background.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

This antibody works

The Bad

Some background

The Bottom Line

Will buy and use it again.

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