Thermo Fisher Scientific
STAT3 Monoclonal Antibody (232209), FITC
MA5-23635
232209
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at test staining STAT3.
Flow Cytometry
Isolated PBMCs from HD peripheral blood
BD Phosflow Lyse/Fix Buffer + PermIII. anti-STAT3 FITC 1/5, 1h RT
5 µl FcR-Blocking
N/A
Flow cytometer
There is positive staining in comparison with the isotype control.
PMID: 32357246
It works
It is not the brightest
Can be used