Usable Anti-pSTAT1

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

Alexa Fluor 488 Mouse Anti-Stat1 (pY701)

Catalog Number:

612596

Clone Number:

4a

Browse Similar Products: Stat1 Flow Cytometry Antibodies

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining pSTAT1.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole blood lysed/ Fixed (BD 558049) + Perm Buffer III (BD 558050)

Primary Incubation

pSTAT-1 (1/10) 1h Room temperature

Blocking Agent

5 µl FcR-Blocking

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

Although is not very bright you can see the shift in comparison with the isotype control.

DOI or PMID #

PMID: 32357246

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

It works.

The Bad

It is not bright.

The Bottom Line

It can be used.

Share your experience with other scientists. Write a Review! »

Join the discussion