Good c-Myc Antibody from Santa Cruz

June 22, 2020

BlueskyReddit
Pharmacology and Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

MYC antibody

Catalog Number:

sc-40

Clone Number:

9E10

I use this antibody to detect MYC tagged protein in the transfected cells by western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Hela cells

Primary Incubation

1;200, 4 degree, overnight

Blocking Agent

5% milk

Secondary Incubation

1:2000 room temp 1 hour

Tertiary Incubation

None

Detection

ECL

Results Summary

I got strong and specific bands for the samples transfected with MYC tagged protein. The background was clean.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong and specific signal

The Bad

None

The Bottom Line

I would recommend this antibody for western blot experiments. It is also recommended by other users in the lab for immunoprecipitation.

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