Good Antibody for pAKT308 from CST

June 24, 2020

Pharmacology and Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-Akt (Thr308) (244F9) Rabbit mAb

Catalog Number:

4056

Clone Number:

244F9

This antibody works very well to detect endogenous AKT phosphorylation at Thr308 when doing western blot with cell lysate.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cancer cell lines

Primary Incubation

1:1000 4 ℃ overnight

Blocking Agent

5% BSA

Secondary Incubation

1:2000 room temp 1 hour

Tertiary Incubation

None

Detection

ECL

Results Summary

I obtained strong and specific bands at correct molecular weight with clean background. Expected change in phosphorylation level was seen with purturbation of the signaling pathway.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Strong and specific signal

The Bad

None

The Bottom Line

This antibody is one of the top choices for phospho-AKT308 which has been used in numerous publications.

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