Good Antibody for EOMES Flow Cytometry

June 10, 2020

Pharmacology and Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

EOMES Monoclonal Antibody (WD1928), eFluor 660

Catalog Number:

50-4877-41

Clone Number:

WD1928

I use this antibody for the cytoflowmetry experiment to detect EOMES positive cell population in my cell sample

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

H9 cell line

Primary Incubation

1:50, room temperature, 45 minutes

Blocking Agent

FACS buffer

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence

Results Summary

EOMES which turns on during embryonic stem cell differentiation to mesendoderm cells, was positively stained in my mesendoderm cells but not in the ES cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong and specific signal

The Bad

None

The Bottom Line

I would recommend this antibody for flow cytometry for EOMES.

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