Poor Signal, Basically Does Not Work

July 01, 2020

BlueskyReddit
Pharmacology and Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

HMGCR Antibody

Catalog Number:

sc-271595

Clone Number:

C1

I used this antibody to detect HMGCR protein in cells undergoing differentiation. The signal was very weak and nonspecific.

Experimental Design and Results Summary

Applications

Western Blot

Sample

H9 cell line

Primary Incubation

1;50, 4 degree, overnight

Blocking Agent

5% milk

Secondary Incubation

1:10000, room temperature, 1 hour

Tertiary Incubation

None

Detection

Fluorescence

Results Summary

The blot was very dirty. There might be some bands at the expected molecular weight, but I was not convinced that was the true signal.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

None

The Bad

Does not work

The Bottom Line

I don't recommend this antibody.

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