Alexa Fluor® 488 anti-Oct4 (Oct3) Antibody
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I used this OCT4 antibody to detect OCT4 expression level in embryonic stem cells and as they differentiate into different lineages.
H9 cell line
1:25, room temperature, 45 minutes
There is strong OCT4 staining in nondifferentiated stem cells, but as they differentiate to neural projenitor cells, OCT4 signal greatly decreased.
Strong and specific signal
This antibody works really well in flow cytometry with human embryonic stem cells and related cell lines.