Great OCT4 Antibody for Flow Cytometry

May 28, 2020

Pharmacology and Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 488 anti-Oct4 (Oct3) Antibody

Catalog Number:

653706

Clone Number:

3A2A20

I used this OCT4 antibody to detect OCT4 expression level in embryonic stem cells and as they differentiate into different lineages.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

H9 cell line

Primary Incubation

1:25, room temperature, 45 minutes

Blocking Agent

FACS buffer

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence

Results Summary

There is strong OCT4 staining in nondifferentiated stem cells, but as they differentiate to neural projenitor cells, OCT4 signal greatly decreased.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Strong and specific signal

The Bad

None

The Bottom Line

This antibody works really well in flow cytometry with human embryonic stem cells and related cell lines.

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