Good GATA3 Antibody for Flow Cytometry

June 03, 2020

Pharmacology and Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-GATA3 Antibody

Catalog Number:

653803

Clone Number:

16E10A23

I use this GATA3 antibody to test the efficiency of embryonic stem cell differentiation to trophoblast cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

H9 cell line

Primary Incubation

1:50, room temperature, 45 minutes

Blocking Agent

FACS buffer

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence

Results Summary

GATA3 worked well as a marker for trophoblast as shown by the flow cytometry result using this antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Strong and specific signal

The Bad

None

The Bottom Line

I would recommend this antibody if you are running flow cytometry experiment for GATA3.

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