ELISA Kit for Measuring Mouse IL-4

June 10, 2020

BlueskyReddit
National Dairy Research Institute
Animal Biochemistry
Scientist

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Company:

Thermo Fisher Scientific- Invitrogen

Product Name:

Mouse IL-4 ELISA kit

Catalog Number:

BMS613

Blood was collected from retro-orbital plexus of eye from mouse and then it was centrifuged. The supernatant was collected as mouse serum and was analysed for the presence of IL-4. The assay was performed according to the manufacturer’s instructions. The ELISA kit is based on sandwich ELISA and the absorbance is measured at 450 nm.

Experimental Design and Results Summary

Application

Detection of Mouse IL-4 in serum

Starting Material

Blood

Tips

Kit components and samples should be brought to room temperature before performing the assay.

Results Summary

The absorbance was measured at 450 nm and the concentration of Mouse IL-4 was calculated from the standard curve. The ELISA kit was very specific and sensitive.

DOI or PMID #

N/A

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Summary

The Good

Performing the assay by using ELISA kit was very convenient and easy. It is very sensitive kit and detected even 2.5 picograms of IL-4 per ml of serum.

The Bad

N/A

The Bottom Line

Sensitive and specific ELISA kit for measuring Mouse IL-4 levels in serum.

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