Great CD271 Antibody

May 29, 2020

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD271 (LNGFR)-FITC, human

Catalog Number:

130-113-420

Clone Number:

ME20.4-1.H4

This antibody was used to visualize the CD271 epression in human skin by direct immunfluorescence. The specimen was fresh frozen and fixed with acetone.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human skin

Primary Incubation

1:20; RT; 60 min

Blocking Agent

5 % FBS; RT, 30 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence signals (FITC) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives specific signal on frozen-tissue sections with little background. Publishable results!

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works also for IF

The Bad

None

The Bottom Line

Will buy and use it again. Recommendable!

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