Great Recombinant CD276 Antibody

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD276-PE, human

Catalog Number:

130-118-698

Clone Number:

REA1094

In this project the pathogensis of melanoma was investigated by a multiplex immunfluorescence technique, where you can stain up to 100 antibodies simultaneously. We analyzed protein expression profiles different stages of melanoma. CD276 was one of our targets.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human skin (melanoma)

Primary Incubation

1:50; RT; 60 min

Blocking Agent

5% FBS; RT, 30 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence signals (PE) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

Very specific and strong signals!

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Reliable results, easy to use

The Bad

None

The Bottom Line

Also suitable for IF

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