Good CD279 Antibody

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD279 (PD1)-PE, human

Catalog Number:

130-120-388

Clone Number:

REA1165

In this project the pathogensis of melanoma was investigated. With the MELC technology, we analyzed protein expression profiles in nevi and melanomas. Here we focused on the PD-1 expression in these conditions.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Melanoma

Primary Incubation

1:40; RT; 60 min

Blocking Agent

5% FBS; RT, 30 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence signals were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives specific signal on frozen-tissue sections with little background. Degree of labeling (DOI) for this antibody is improvable. Publishable results!

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

This antibody works.

The Bad

Degree of labeling for this antibody is improvable.

The Bottom Line

Will buy and use it again.

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