Tested for IF - Good CD206 Antibody

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD206-FITC, human

Catalog Number:

130-123-920

Clone Number:

DCN228

In this project the different phenotypes of immune cells in lymph nodes were investigated by a multiplex immunfluorescence technique. CD206 was one of many marker in our analysis.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Lymph node human

Primary Incubation

1:20; RT; 60 min

Blocking Agent

5% FBS; RT, 30 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence signals (FITC) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives specific signal on frozen-tissue sections with little background. Degree of labeling (DOI) for this antibody is improvable.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

This antibody works

The Bad

None

The Bottom Line

Will buy and use it again. Recommendable!

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