Great CD205 Antibody from Miltenyi

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD205 (DEC205)-PE, human

Catalog Number:

130-096-369

Clone Number:

HD30

Our lab was interested in the investigation of the activation of ADAM proteases and their substrates in human skin. By using a multiplex immunfluorescence technique we could stain up to 100 antigen on one single tissue slide. Also we stained for antigens which are present on Langerhans cell, like C205.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human skin

Primary Incubation

1:10; RT; 60 min

Blocking Agent

5% FBS; RT, 30 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence signals (PE) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives specific signals on frozen-tissue sections with little background. Publishable results!

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific staining

The Bad

Little background

The Bottom Line

Will buy and use it again. Recommendable!

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