Good CD209 Antibody for IF

May 20, 2020

BlueskyReddit
Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD209 (DC-SIGN)-FITC, human

Catalog Number:

130-092-873

Clone Number:

DCN47.5

Our lab was interested in the charaterization of leukocytes in psoriasis and eczema in order to find different subtypes of immune cells for a better differentiation of these two diseases.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human skin (psoriasis)

Primary Incubation

1:20; RT; 60 min

Blocking Agent

5% FBS; RT, 30 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence signals (FITC) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives specific signal on frozen-tissue sections with background. Degree of labeling (DOI) for this antibody is improvable. Publishable results!

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

This antibody works

The Bad

Some background staining

The Bottom Line

This antibody is also suitable for IF.

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