Very Good CD276-Antibody for IF

May 19, 2020

BlueskyReddit
Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD276-FITC, human

Catalog Number:

130-095-527

Clone Number:

FM276

In this project the different phenotypes of immune cells in human skin diseases were investigated by a multiplex immunfluorescence technique. On these image you see an specific CD276 of a cryo-tissue section (psoriasis).

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human skin (psoriasis)

Primary Incubation

1:20; RT; 60 min

Blocking Agent

5% FBS; RT, 30 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence signals (FITC) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives specific signal on frozen-tissue sections with little background.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

This antibody works

The Bad

None

The Bottom Line

Also a great antibody for IF (not only FC).

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