Great Flow Antibody to Detect MHC Class II Expression on Cells

June 10, 2020

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 711™ anti-mouse I-A/I-E Antibody

Catalog Number:

107643

Clone Number:

M5/114.15.2

We are an immunology lab that looks at immune responses in the context of inflammation and cancer. This marker is used to widely used to identify proper antigen presentation of Class II on antigen presenting cells in flow cytometry experiments. Expression of MHC II indicates of mature activated antigen presenting cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Splenocytes (Naive and Infected)

Primary Incubation

Multiple Dilutions (1:250, 500, 1000)

Blocking Agent

FC Block 100ng/mL in FACS solution (surface)

Secondary Incubation

None

Tertiary Incubation

None

Detection

Cytek Aurora (BVR)

Results Summary

Splenic cells are strongly and specifically labelled with MHC Class II to identify populations with low and high expression to mark experienced antigen presenting cells.

DOI or PMID #

N/A

Additional Notes

Accordingly Titrate Antibody based on flow analyzer.

Related Categories

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Summary

The Good

Reliable results as easy to idenitify MHC Class II positive cells from other spenic cells.

The Bad

None

The Bottom Line

I would recommend this antibody to anyone analyzing antigen presenting cells. It gives a strong, consistent labeling of MHC Class II positive cells for flow cytometry analysis.

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