Good Antibody to Detect CD137 Surface Expression using Flow Cytometry

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

CD137 (4-1BB) Monoclonal Antibody (17B5), PerCP-eFluor 710

Catalog Number:

46-1371-82

Clone Number:

(17B5)

Browse Similar Products: CD137 Flow Cytometry Antibodies

We are an immunology lab that looks at immune responses in the context of inflammation and cancer. This marker is used to widely used to identify CD137+ T cells in flow cytometry experiments especially in the tumor setting.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Splenocytes (Naive and Infected)

Primary Incubation

Multiple Dilutions (1:250, 500, 1000)

Blocking Agent

FC Block 100ng/mL in FACS solution (surface)

Secondary Incubation

None

Tertiary Incubation

None

Detection

Cytek Aurora (BVR)

Results Summary

We are able to detect a nice population of CD137+ cells in the spleen.

DOI or PMID #

N/A

Additional Notes

Accordingly Titrate Antibody based on flow analyzer.

Related Categories

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Summary

The Good

Easy to idenitify CD137+ cells from other spenic cells.

The Bad

Need higher concentration of antibody to use

The Bottom Line

Recommend this antibody to detect CD137 in this flow color which is typically dim to begin with.

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