Good Antibody for Phospho-PLCgamma1 from CST

June 23, 2020

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-PLCγ1 (Tyr783) Antibody #2821

Catalog Number:

2821

Clone Number:

Polyclonal

We are interested in how mTOR signaling regulates lymphocyte differentiation.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lymphocyte lysates

Primary Incubation

1:1000 in 4% BSA

Blocking Agent

5% milk

Secondary Incubation

1:5000 in 5% milk

Tertiary Incubation

None

Detection

ECL

Results Summary

Relatively good signal upon immediate TCR activation.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Relative good signal

The Bad

None

The Bottom Line

Might need to titrate before use.

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