Very Good Antibody for H3

June 25, 2020

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Histone H3 (D1H2) XP® Rabbit mAb #4499

Catalog Number:

4499

Clone Number:

D1H2

We are interested in mTOR signaling and how it regulates lymphocyte differentiation.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lymphocyte lysates

Primary Incubation

1:10000 in 4% BSA

Blocking Agent

5% milk

Secondary Incubation

1:5000 in 5% milk

Tertiary Incubation

None

Detection

ECL

Results Summary

Very bright signal

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very good signal

The Bad

None

The Bottom Line

Can be reused multiple times. Definitely worth buying it.

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