V500 Rat Anti-Mouse CD45

June 05, 2020

Innate Immunity
DRFZ
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

V500 Rat Anti-Mouse CD45

Catalog Number:

561487

Clone Number:

30-F11

Browse Similar Products: CD45 Flow Cytometry Antibodies

This antibody was used to identify tissue resident immune cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine brain tissue

Primary Incubation

20mins 37oC

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS BD LSRFortessa, software FACSDiva

Results Summary

Nice antibody specific for CD45 expressing cells. Could identify CD45high and intermediate cells. The resolution could be better.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific, could distinguish between CD45high and CD45int cells

The Bad

Moderate specificity

The Bottom Line

I would recommend this antibody

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