Great Antibody for IP Application

June 15, 2020

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Myc-Tag (9B11) Mouse mAb #2276

Catalog Number:

2276

Clone Number:

9B11

We are trying to study protein interaction by overexpression Myctag fusion protein.

Experimental Design and Results Summary

Applications

Immunoprecipitation

Sample

293T lysate

Primary Incubation

1:1000 in 4% BSA

Blocking Agent

5% milk

Secondary Incubation

1:5000 in 5% milk

Tertiary Incubation

None

Detection

ECL

Results Summary

We are able to detect enrichment of Myctag-fusion protein compared to input.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Very good antibody

The Bad

None

The Bottom Line

Very good IP antibody

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