Good Antibody for Phospho-FOXO1 from CST

June 12, 2020

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technolgoy

Product Name:

Phospho-FoxO1 (Ser256) Antibody #9461

Catalog Number:

9461

Clone Number:

Polyclonal

We are interested in studying mTOR signaling and T cell differentiaton.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lymphocyte lysate

Primary Incubation

1:2500 in 4% BSA

Blocking Agent

5% milk

Secondary Incubation

1:5000 in 5% milk

Tertiary Incubation

None

Detection

ECL

Results Summary

Nice detection of the phosphorylation upon T cell activation.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good detection

The Bad

None

The Bottom Line

Another reliable antibody from Cell Signaling.

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