Decent ID2 Antibody

June 08, 2020

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Thermo Fisher Scientific

Product Name:

ID2 Monoclonal Antibody (ILCID2), PE, eBioscience™

Catalog Number:

12-9475-82

Clone Number:

ILCID2

We are trying to study CD8 T cell differentiation in response to viral infection.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

Fc block

Blocking Agent

FACS buffer

Secondary Incubation

Surface stain

Tertiary Incubation

ICS for 1hr at RT

Detection

Flow cytometry

Results Summary

Able to detect positive staining, but it is not super bright

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Still able to distinguish positive shift

The Bad

None

The Bottom Line

As long as there is control to distinguish positive and negative, it is still a good clone to use.

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