Decent Antibody for TNFa from BioLegend

June 01, 2020

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-mouse TNF-α Antibody

Catalog Number:

506328

Clone Number:

MP6-XT22

We are trying to study CD8 T cell differentiation in response to viral infection.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

Fc block

Blocking Agent

FACS buffer

Secondary Incubation

Surface stain

Tertiary Incubation

ICS at RT for 1hr

Detection

Flow cytometry

Results Summary

We were able to detect TNF and IFNg double positive cell upon ex vivo restim.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Nice staining

The Bad

None

The Bottom Line

Bright fluorochrome for TNF

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