Great Antibody for CD127 from BioLegend

May 20, 2020

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-mouse CD127 (IL-7Rα) Antibody

Catalog Number:

135027

Clone Number:

A7R34

We are trying to study the differentiation of CD8 T cells in response to viral infection.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Spleen

Primary Incubation

Fc block

Blocking Agent

FACS buffer

Secondary Incubation

Surface stain for 20min at 4C

Tertiary Incubation

Fix

Detection

Flow cytometry

Results Summary

Very good staining for CD127.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very bright staining

The Bad

None

The Bottom Line

Good antibody combination for CD127.

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