New Flourchrome to Detect CD4 T Cells Using Flow Cytometry

May 12, 2020

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Spark Blue™ 550 anti-mouse CD4

Catalog Number:

100473

Clone Number:

GK1.5

We are an immunology lab that looks at immune responses in the context of inflammation and cancer. This marker is used to widely used to identify CD4 T cells in flow cytometry experiments. This new color will nicely fit into the blue laser of our Aurora analyzer.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Splenocytes (Naive and Infected)

Primary Incubation

Multiple Dilutions (1:250, 500, 1000)

Blocking Agent

FC Block 100ng/mL in FACS solution (surface)

Secondary Incubation

None

Tertiary Incubation

None

Detection

Cytek Aurora (BVR)

Results Summary

Splenic CD4 T cells are strongly and specifically labelled to identify the population with CD4 SparkBlue 500 even during an infection comparable to naive cells.

DOI or PMID #

N/A

Additional Notes

Accordinly Titrate Antibody based on flow analyzer.

Related Categories

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Summary

The Good

Reliable results as easy to idenitify CD4+ T cells from other spenic cells.

The Bad

None

The Bottom Line

I would recommend this new antibody to anyone studying T cells. It gives a strong, consistent labeling of CD4 T cells that may fit nicely into your panel if carefully designed.

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