Anti-Nestin Stains Tissue Very Well

May 21, 2020

Genetics
Emory University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Nestin Antibody

Catalog Number:

ab22035

Clone Number:

10C2

The anti-Nestin antibody works very strongly on tissue slices.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Organoid

Primary Incubation

1:1000 at 4C overnight

Blocking Agent

5% donkey serum and 0.01% Triton-X100 in PBS

Secondary Incubation

Anti-mouse AlexaFlor-594 1:1000 at RT for 2 hours

Tertiary Incubation

N/A

Detection

Fluorescent Microscope

Results Summary

Beautiful stains. Comes out high specialized and very strong signal.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Stains strongly

The Bad

N/A

The Bottom Line

Works really well. I would recommend.

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