A Miniprep Kit for When You Need Extremely Highly Pure DNA

Havard Medical School
Dpt of Cell Bio
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

PureLink™ HiPure Plasmid Miniprep Kit

Catalog Number:

K2100-02

I use this kit only when I need very pure DNA. Otherwise, I use an Omegabiotech mini-prep kit for the usual DNA miniprep.

Experimental Design and Results Summary

Application

DNA miniprep, especially to get very pure DNA (A260/280 >1.8) for next generation sequencing samples

Starting Material

Cells

Tips

The protocol is very straight forward.

Results Summary

When I isolate the DNA using this kit, I consistently get A260/A280 > 1.80. But this is quite expensive compared to the ordinary PCR kits, so I use this kit for special experiments such as NGS.

DOI or PMID #

N/A

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Summary

The Good

Good quality of the end product (DNA), and consistent results

The Bad

Expensive

The Bottom Line

It is good to have one for the lab to share for rare occasions.

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