A Starvation Medium for Imaging Analysis and Autophagy Study

Havard Medical School
Dpt of Cell Bio
Postdoctoral Researcher

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Ease-of-Optimization

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Company:

Sigma Aldrich

Product Name:

Earle’s Balanced Salts

Catalog Number:

E3024-500ML

This medium can induce autophagy through starvation, and it can maintain pH under 5% CO2 condition.

Experimental Design and Results Summary

Application

Tissue culture cell starvation

Starting Material

Tissue culture

Tips

Pre-warm the media before adding it to cell

Results Summary

This media worked well when I performed long-term live-cell imaging analysis with CO2 incubation chamber. The pH of the medium was well maintained.

DOI or PMID #

N/A

Related Categories

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Summary

The Good

Simple to use

The Bad

Nothing

The Bottom Line

This media induces autophagy within 30 minutes.

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