Sensor for Acidic Lysosome

Havard Medical School
Dpt of Cell Bio
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

LysoSensor™ Green DND-189

Catalog Number:

L7535

This green fluorescent dye gives a signal when it goes to an acidic lumen of the lysosome. It works quite well in my hands.

Experimental Design and Results Summary

Application

Live cell imaging of lysosomes

Starting Material

N/A

Tips

I add 1 micro molar concentration of the dye 30 minutes before the live cell imaging.

Results Summary

This probe works well to stain highly acidic lysosomes in live cells. It gives a strong green fluorescent signal only when it goes to an acidic compartment of the cell.

DOI or PMID #

N/A

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Summary

The Good

Sensitive and last for very long

The Bad

Nothing

The Bottom Line

If you study lysosomes, this probe will be useful.

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