Very Good Quality MOPS Buffer for SDS-PAGE Using Bis-Tris Gels

Havard Medical School
Dpt of Cell Bio
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

NuPAGE™ MOPS SDS Running Buffer (20X)

Catalog Number:

NP000102

My lab members share this buffer for SDS-PAGE especially when using NUPAGE Bis-tris gels to resolve high molecular weight proteins. So far, the product quality has been consistently good, and we are happy that it has long shelf-life.

Experimental Design and Results Summary

Application

SDS-PAGE running buffer

Starting Material

N/A

Tips

Keep the 1X buffer at 4 degree to prevent the heat-up during electrophoresis

Results Summary

My lab members have been using this buffer together with MES buffer depends on the size of the protein of interest. We are pleased with this invitrogen product as it saves us tremendous time.

DOI or PMID #

N/A

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Summary

The Good

Convenient and consistently good quality. No color change over time

The Bad

Nothing

The Bottom Line

I recommend this product! If many people use MOPS buffer, it's worth buying this big batch product.

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