Fc Receptor Blocker Is Great for IHC

UAB
Radiation Oncology
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Innovex Biosciences

Product Name:

Fc Receptor Blocker

Catalog Number:

NB309

Fc Receptor Blocker was used to block non-specific Fc receptor on mouse breast tumor tissue specimens for IHC. Better signaling of target antibodies is detected as a result.

Experimental Design and Results Summary

Application

Immunohistochemistry (Immunofluorescence)

Starting Material

Mouse breast tumor tissue

Tips

Use pipet to minimize waste of Fc Receptor Blocker rather than the dropper

Results Summary

Fluorescence detected was reliable and specific to the target antibodies analyzed in IHC. Fluorescence was also maintained (without loss) after months of storage.

DOI or PMID #

N/A

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Summary

The Good

Easy to use

The Bad

N/A

The Bottom Line

The Fc Receptor Blocker should be used prior to Background Buster in order to minimize antibody cross-reactivity with other epitopes. This reagent is highly recommended in order to obtain reliable results in animal tissues for IHC.

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