Perfect Antibody for FLAG Tag

May 12, 2020

BlueskyReddit
Pharmacology and Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

MilliporeSigma

Product Name:

Monoclonal ANTI-FLAG® M2 antibody produced in mouse

Catalog Number:

F1804-200UG

Clone Number:

M2

Browse Similar Products: FLAG Western Blot Antibodies

I use this antibody to do western blot for detecting FLAG tagged proteins in the cell. It works very well.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HEK293T

Primary Incubation

1:2000, 4 ℃, overnight

Blocking Agent

5% milk

Secondary Incubation

1:10000, room temperature, 1 hour

Tertiary Incubation

None

Detection

Fluorescence

Results Summary

I detected very strong and clean bands for the proteins tagged with FLAG tag.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong and specific signal

The Bad

None

The Bottom Line

This is one of the best antibodies with high specificity and high affinity. It works very well for proteins with FLAG tag.

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