Good Antibody for CAND2

Pharmacology and Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Bethyl Laboratories Inc

Product Name:

CAND2 antibody

Catalog Number:

A304-046A

Clone Number:

Polyclonal

Browse Similar Products: CAND2 Western Blot Antibodies

I use this antibody to detect CAND2 by western blot. The abundance of CAND2 is low in cells, many antibodies we tested did not work except this one from Bethyl. I detected band at expected molecular weight, although there are non-specific bands.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HEK293T

Primary Incubation

1:1000 4 ℃ overnight

Blocking Agent

5% milk

Secondary Incubation

1:10000, room temperature, 1 hour

Tertiary Incubation

None

Detection

Fluorescence

Results Summary

Band at expected molecular weight was detected. Specificity was confirmed by the CAND2 CRISPR knockout cell line.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific band with good signal.

The Bad

Nonspecific bands were also detected

The Bottom Line

I would recommend this antibody for western blot analysis, since it seems to be better than the ones we tested from other companies.

Share your experience with other scientists. Write a Review! »

Join the discussion