PDI Antibody: Very Good ER Marker

Molecular Biology
Princeton
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

PDI (C81H6) Rabbit mAb

Catalog Number:

3501

Clone Number:

C81H6

Excellent marker for ER localization, worked perfectly the first time.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

MDA-MB-231 BrCA cells

Primary Incubation

1:500

Blocking Agent

5% goat serum

Secondary Incubation

1:2000 AL647

Tertiary Incubation

None

Detection

Confocal fluorescence

Results Summary

Results showed excellent ER stain with minimal background staining. Worked first try.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Excellent signal to noise ratio, works great at 1:500

The Bad

None

The Bottom Line

Excellent ER marker, no titration needed.

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