Excellent LAMP1 Antibody for Lysosomal Stain

May 12, 2020

BlueskyReddit
Molecular Biology
Princeton
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

LAMP1 (D2D11) XP® Rabbit mAb

Catalog Number:

9091

Clone Number:

D2D11

Very good signal to noise for lysosomal stain. Stain was broader than expected, but antibody appeared to be excellent.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

MDA-MB-231 BrCa cells

Primary Incubation

1:500

Blocking Agent

5% goat serum in PBS-T

Secondary Incubation

1:2000 AL647

Tertiary Incubation

None

Detection

Confocal scanning

Results Summary

Results showed very good signal to noise but very broad staining beyond what was expected. No titration was needed beyond manufacturer's suggestion.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Excellent staining

The Bad

No negatives

The Bottom Line

Very good lysosomal stain, didn't need to try any other markers.

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